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Barking up the wrong tree is an idiomatic expression in English, which is used to suggest a mistaken emphasis in a specific context. The phrase is an allusion to the mistake made by dogs when they believe they have chased a prey up a tree, but the game may have escaped by leaping from one tree to another. The phrase means to mistake one's object, or to pursue the wrong course to obtain it.
In other words, "if you are barking up the wrong tree, it means that you have completely misunderstood something or are totally wrong."